Formulation of asiaticoside obtained from centella asiatica in microemulsion gel

Abstract

This study was aimed to develop microemulsion gels containing asiaticoside from Centella asiatica. Presently purified asiaticoside is needed in cosmetic and pharmaceutical formulations. But mostly used just crude extract from ethanol with low content of asiaticoside. In this study asiaticoside was isolated from fresh C. asiatica leaves by extraction with ethanol, hexane, chloroform, and buthanol. Asiaticoside was purified by gel-filtration chromatography which using Sephadex LH-20 as the stationary phase and recrystallization from ethanol. The isolation & characterisation of asiaticoside were done by using high performance liquid chromatorgraphy (HPLC) & high resolution liquid chromatoraphy-mass spectrometry (LC-MS) with electrospray ionisation. Six formulations of asiaticoside with different oil component and surfactant of microemulsion gel were prepared to evaluate the best formulationl Microemulsion gel containing asiaticoside was comprised of capric/caprylic triglyceride : polyoxyethylene 10 olyl ether (Brij 97) : distilled water : asiaticoside = 15 : 70 : 14 : 1. This formulation penetrated through shed snake skin in modified Franz diffusion cell with higher flux value than other microemulsion gel formulation.