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Now showing items 1-20 of 188

Accumulation of glycine betaine and partial purification of betaine aldehyde dehydrogenase from a halotolerant cyanobacterium aphanothece halophytica

Uthaiwon Kumarb (Chulalongkorn University, 1997)

The H1-NMR spectroscopy was a suitable method for the determination of glycine betaine in a halotolerant cyanobacterium, A.halophytica. The amount of glycine betaine from A.halophytica was 9.7 nmol/10 6 cells when the cells ...
The active site of cyclodextrin glycosyltransferase from Bacillus sp. A11

Anchalee Tongsima (Chulalongkorn University, 1998)

Chemical modification of CGTase from Bacillus sp. A11 was performed under optimized mild conditions. Loss of CGTase activities was observed when modifications were made on carboxyl, histidine, tryptophan, and tyrosine ...
Alanine production by Escherichia coli transformed with alanine dehydrogenase and formate dehydrogenase genes

Rujirat Hatrongjitt, 1979- (Chulalongkorn University, 2004)

Alanine dehydrogenase (EC 1.4.1.1) catalyzes the NAD[superscript +]-dependent reversible oxidative deamination of L-alanine to form ammonia, pyruvate, and NADH. The enzyme is important as a catalyst for the synthesis of ...
Altering substrate specificity of alpha-amylase by bioimprinting with beta-cyclodextrin

Pisit Sirisuwan (Chulalongkorn University, 2007)

The aim of this study was to alter the substrate specificity of ∞-amylase by the combination of bioimprinting with ®-cyclodextrin (®-CD) and immobilization method. ∞-Amylase was first derivatized with itaconic anhydride ...
Antioxidant and anti-melanogenesis properties of guava (Psidium guajava L.) extracts in vitro

Thanakorn Damsud (Chulalongkorn University, 2008)

Guava (Psidium guajava L.) is a tropical fruit, rich in nutrients such as vitamin C, phenolic compounds, pectin and insoluble fiber. We focus on Pansithong, Thai original and Kimju grown locally commercial in Thailand by ...
Antiviral responses and biological targets of a kazal-type serine proteinase inhibitor spipm2 from the black tiger shrimp Penaeus monodon

Sirikwan Ponprateep (Chulalongkorn University, 2013)

White spot syndrome (WSS) is a severe viral disease in Penaeus monodon which causes high mortality. The understanding of shrimp immunity will lead to the knowledge for prevention and solving the disease. In black tiger ...
Association effect of nitrogen-fixing Klebsiella sp. and rice on rice lectin

Chetsadaporn Pitaksutheepong (Chulalongkorn University, 1992)
Binding properties and crystallization of crustinPm1 and crustinPm7 from tha black tiger shrimp penaeus monodon

Pasapong Poolpipat (Chulalongkorn University, 2010)

Crustin is one of several antimicrobial peptides (AMPs) in innate immune system of crustacean. In previous study, crustinPm1 strongly inhibited gram-positive bacteria, whilst crustinPm7 acted against both gram-positive and ...
Biochemical and structural characterization of levansucrase from bacillus licheniformis RN-01

Santhana Nakapong (Chulalongkorn University, 2011)

Levansucrase (Ls) is a fructosyltransferase belonging to family 68 of glycosyltransferase using sucrose as a sole substrate to synthesize levan, a fructose polymer. Bacillus licheniformis RN-01, isolated from soil in ...
Biodiversity of uncultured Streptomyces involved in bioactive substance production from soil in Thailand

Sirinee Yodmuang (Chulalongkorn University, 2008)

Streptomyces, gram-positive filamentous bacteria and G-C rich nucleotide, have capability of producing several bioactive compounds and predominantly exhibit in soil. This study is aimed to investigate the genetic diversity ...
Bioinformatic analysis of Mycoplasma Pneumoniae virulence factors with a Potential role in human brain invasion

Vasunun Chumchua (Chulalongkorn University, 2006)

This investigation was conducted to identify virulence factors of Mycoplasma pneumoniae from its genome by bioinformatic schemes and to determine their affinities on plasminogen which circulate in blood brain vessel, by ...
Changes of polyamines content under osmotic and UV-radiation stresses in cyanobacterium Synechocystis sp. PCC 6803

Apiradee Pothipongsa (Chulalongkorn University, 2009)

Effect of salt and sorbitol at various concentrations and combined with UV radiations, including UV-A (365 nm; 11-13 w/m2), UV-B ( 302 nm; 1.2-1.3 w/m2) and UV-C (254 nm; 1.0-1.3 w/m2), for 3 hours on cell growth, intracellular ...
Characterization and cDNA cloning of major royal jelly proteins of Apis cerana in Thailand

Duangporn Srisuparbh (Chulalongkorn University, 2002)

The large subunit of the ribosomal (Ir) RNA gene of Thai hive bee, Apis cerana was PCR-amplified and sequenced. Based on these nucleotide sequences, genetic differentiation analysis of A. cerana was simplified to a PCR-RFLP ...
Characterization and functional analysis of prophenoloxidase system-associated genes from the black tiger shrimp penaeus monodon / Walaiporn Charoensapsri

Walaiporn Charoensapsri (Chulalongkorn University, 2010)

The prophenoloxidase (proPO) activating system plays an essential role in the immune defence against microbial infections in many invertebrates. In the present study, two PPAE genes (designated PmPPAE1 and PmPPAE2) and a ...
Characterization and gene organization of anti-lipopolysaccharide factor in the black shrimp Penaeus monodon

Sirinit Tharntada (Chulalongkorn University, 2007)

Different isoforms of the ALF homologues (ALFPm1 to 5) have been previously identified from Penaeus monodon expressed sequence tag (EST) database (http://pmonodon.biotec.or.th). The nucleotide and amino acid sequences of ...
Characterization of a novel amylomaltase from corynebacterium glutamicum ATCC 13032

Wiraya Srisimarat (Chulalongkorn University, 2010)

To search for a novel amylomaltase and to identify residue involved in formation of cyclic oligosaccharide product. The amylomaltase gene from Corynebacterium glutamicum ATCC 13032 was cloned and expressed in Escherichia ...
Characterization of a novel protein containing TBC1 domain in the black tiger shrimp Penaeus monodon and its role in reponse to white spot syndrome virus infection

Wanchart Yingvilasprasert (Chulalongkorn University, 2011)

TBC domain (TRE2/BUB2/CDC16) is a 200-amino-acid protein containing a Rab-GTPase-activating protein (GAP) homology domain required for regulating the activity of Rab proteins which are center of endocytic membrane trafficking ...
Characterization of a polymine-binding protein in cyanobacterium Synechocystis sp. PCC 6803

Panutda Youdsang (Chulalongkorn University, 2010)

Synechocystis sp. PCC 6803 PotD protein involved in polyamine transport was overexpressed in Escherichia coli as His-tagged PotD (rPotD). The purified rPotD showed saturable binding kinetics with radioactively-labeled ...
Characterization of Chitinase and Partial Gene Cloning from Microbacterium sp. TU05

Janjaras Sermsatanaswadi (Chulalongkorn University, 2001)

A bacterium TU05 was isolated from soil in Bangkok, Thailand. It can produce chitinase (EC. 3.2.1.14) that catalyzes the degradation of chitin. Gram-stain, biochemical characteristics, and sequence of 16S rRNA gene, ...
Characterization of lead-binding protein in human serum

Suphitcha Mangkalee (Chulalongkorn University, 1994)

Although lead is one of the most useful metals, it is also toxic for human. It may cause anemia and other serious symtoms. Biochemical mechanisms in lead poisoning is of interest. The studies about lead transportation, its ...

Now showing items 1-20 of 188

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