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Acceptor specificity and transglucosylation reaction of amylomaltase from Corynebacterium glutamicum

Wachiraporn Naumthong (Chulalongkorn University., 2013)

Amylomaltase, a 4-α-glucanotransferase, catalyzes intramolecular transglucosylation reaction producing large-ring cyclodextrins (LR-CDs) and intermolecular transglucosylation reaction in which glucosyl units of starch or ...

ANALYSIS OF ANTIOXIDANT SYSTEM IN TRANSGENIC 'KDML105' RICE Oryza sativa L. OVEREXPRESSING OsCaM1-1 UNDER SALINITY STRESS

Taviporn Kaewneramit (Chulalongkorn University, 2014)

To investigate various parameters (growth, physiological and biochemical responses) associated with improved tolerance under salt stress in the transgenic rice overexpressing OsCaM1-1 gene, the control and the wild-type ...

Antibacterial activity and gene regulation of antilipopolysaccharidefactor from black tiger shrimp Penaeus monodon

Pitchayanan Kamsaeng (Chulalongkorn University, 2014)

Antilipopolysaccharide factor (ALF) is one of the antimicrobial peptide families identified in invertebrates. Six different isoforms of the ALF homologues have been identified from Penaeus monodon. Previously, ALFPm6 has ...

Anticancer activity of mansonone G derivatives against human non-small cell lung cancer: computational and mechanistic study

Panupong Mahalapbutr (Chulalongkorn University, 2018)

Epidermal growth factor receptor (EGFR) and DNA topoisomerase II (Topo II) are the key molecular targets for non-small cell lung cancer (NSCLC) due to its major contribution to complex signaling cascades modulating the ...

Cam-binding property and roles in stress response of myosin heavy chain in rice oryza sativa L.

Jutamas Anantayanon (Chulalongkorn University, 2014)

When plants encounter to environmental stimuli both biotic and abiotic stresses, plants will response using the signal transduction pathway by rapid increasing the concentration of calcium ion in the cytosol of plant cell. ...

CHARACTERIZATION OF GLUTAMATE DECARBOXYLASE IN Synechocystis sp. PCC 6803 AND ITS ROLES IN RESPONSE TO ABIOTIC STRESS

Simab Kanwal (Chulalongkorn University, 2013)

Glutamate decarboxylase (GAD) is a pyridoxal-5′-phosphate dependent enzyme that catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA) through irreversible decarboxylation reaction. GAD is widely distributed ...

Cloning and characterization of bacterial mannanase isolated from dynastes hercules larvae

Sitipon Leerawatthanakun (Chulalongkorn University, 2017)

Bacterial isolate HM7 was isolated from Dynastes hercules larvae excrement. It was identified as Bacillus sp. by 16S rRNA gene analysis. When the bacteria were cultured for β-mannanase production in medium containing konjac ...

Controlled release of fibroin repeat-tagged proteins from silk hydrogel

Jaturong Promsuk (Chulalongkorn University, 2020)

Protein-based biologic drugs such as growth factors are playing important roles in modern medicine. These proteins can be used to stimulate local tissue repair. Growth factors are often incorporated into a scaffold, such ...

Designing peptides with inhibitory activity on human papillomavirus 16 E1-E2 protein complex formation

Worrapon Kantang (Chulalongkorn University, 2015)

Human papillomavirus 16 (HPV 16) is a DNA virus that is capable of infecting humans and causing cervical cancer. HPV16 E2 plays an important role in viral gene regulation and replication. Fujii et al 2003 reported that ...

Drug resistance mechanism of ns3/4a protease and identification of potent compounds inhibiting reverse transcriptase of hepatitis virus

Jirayu Kammarabutr (Chulalongkorn University, 2018)

Viral Hepatitis has become a public health concern which caused 1.34 million deaths in 2015 and 95% of sickness and untimely deaths come from chronic hepatitis B and C virus infections. The epidemic of viral hepatitis B ...

Effect of feedback-resistant aroG overexpression on L-phenylalanine production in Escherichia coli

Maria Ulfah (Chulalongkorn University, 2018)

L-Phenylalanine (L-Phe) is one of the most important amino acids in food and pharmaceutical industries. It is widely used as a nutritional supplement and a precursor for the synthesis of food additives. The market of L-Phe ...

Effect of nutrient modification on lipid level in cyanobacterium synechocystis sp. Pcc 6803 overexpressing aas gene

Kamonchanock Eungrasamee (Chulalongkorn University, 2015)

In this study, Synechocystis sp. PCC 6803 strain overexpressing aas gene (Ox-Aas) was successfully constructed via single recombination. The putative acyl-acyl carrier protein synthetase (AAS) is a key enzyme in fatty acid ...

Effects of mutations of levansucrase from Bacillus licheniformis RN-01 on levan oligosaccharides binding

Thassanai Sitthiyotha (Chulalongkorn University, 2018)

Produced by levansucrase that uses sucrose as a substrate, levan and levan oligosaccharides (GFn) have potential applications in food and pharmaceutical industries such as prebiotics, anti-obesity and anti-tumor agents. ...

ENHANCED PRODUCTION OF L-AMINOADIPIC ACID IN Escherichia coli BY METABOLIC ENGINEERING OF LYSINE AND ADIPIC ACID BIOSYNTHESIS

Teerapat Norasetsingh (Chulalongkorn University, 2016)

L- Aminoadipic acid (L- AAA), a non- protein structure amino acid, is an important intermediate for many medicinal compounds such as antirheumatic drug, psoriasis and carcinostatic drug as well as a precursor in the ...

ENHANCEMENT OF WATER SOLUBILITY OF HESPERETIN AND NARINGENIN BY COMPLEXATION WITH β-CYCLODEXTRIN AND ITS DIMETHYL DERIVATIVE

Waratchada Sangpheak (Chulalongkorn University, 2013)

Hesperetin and naringenin, the flavonoids of the class flavanones, are abundant in citrus fruits. They are widely applied in food products to enhance the health function as well as used in developing nutraceutical and ...

Enzymatic and computational analysis of large-ring cyclodextrin production by Corynebacterium glutamicum amylomaltase

Sirikul Ngawiset (Chulalongkorn University, 2016)

Amylomaltase is a well-known glucanotransferase enzyme that can produce large-ring cyclodextrins (LR-CDs) via cyclization reaction. To determine important amino acid residues involved in LR-CDs production of amylomaltase ...

Expression and characterization of dextransucrase from leuconostoc citreum ABK-1

Kakuttapun Bulan (Chulalongkorn University, 2021)

Dextransucrase is an enzyme in the glycoside hydrolase 70 family that catalyze the hydrolysis of sucrose to fructose and glucose, the glucose residues are transferred to synthesize dextran via transglycosylation reaction. ...

EXPRESSION AND CHARACTERIZATION OF α-GLUCOSIDASE FROM Weissella confusa BBK-1

Lalita Silapasom (Chulalongkorn University, 2017)

Alpha-glucosidase [EC 3.2.1.20; α-D-glucoside glucohydrolase] is an exohydrolase which catalyzes non-reducing end of substrates to release D-glucose. In addition, alpha-glucosidase also displays a transferase activity, ...

FATTY ACID AND LIPID LEVELS IN CYANOBACTERIUM Synechocystis sp. PCC 6803 MUTANT OVEREXPRESSING plsX GENE

Nutchaya Songruk (Chulalongkorn University, 2015)

In this study, we successfully constructed the plsX- overexpressing strain of Synechocystis sp. PCC 6803 (OX_plsX strain), and further studied the effects of salt stress and nutrient modifications on growth, pigment contents ...

Fructooligosaccharide synthesis by immobilized inulosucrase from lactobacillus reuteri 121 and improvement of product pattern using site-directed mutagenesis

Thanapon Charoenwongpaiboon (Chulalongkorn University, 2019)

Inulin-type fructooligosaccharides (IFOS) are well-known prebiotics which can be produced from sucrose using bacterial inulosucrase (E.C. 2.4.1.9). However, the use of inulosucrase for IFOS synthesis has some limitations ...

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