Abstract:
For detection of gene amplification, CISH has all the advantages of FISH but in addition, needs no special microscopy or image capturing systems, and preparations are permanent. In the case of neuroblastoma,
CISH has disclosed considerable heterogeneity in MYCN copy number between cells in a tumour. Heterogeneity
reflects different tumour clones and its role has been under-recognized in neuroblastoma biology. Additional
studies are needed to investigate the significance of tumour heterogeneity in neuroblastoma, and whether the
aggressive (i.e., MYCN-amplified) clones are more likely to metastasize, survive treatment modalities, and ultimately kill the patient.
