Acetylcholinesterase inhibitors from roots of Feroniella lucida

Abstract

The chemical study of acetylcholinesterase (AChE) inhibitory activity compounds from BuOH crude extract from roots of Feroniella lucida in the family Rutaceae led to the isolation of four new furanocoumarins, named feronielloside, feroniellic acid A, feroniellic acid B and feroniellic acid C along with twelve known coumarins, glucopsoralen, bergaptol-O-beta-D-glucopyranoside, aesculetin glycoside, 2", 3"-epoxyanisolactone, anisolactone, marmesin, feroniellin A, feroniellin C, xanthoarnol, gosferol, 7-demethylsaberosine and haploperoside. Their structures were elucidated by the combination of spectroscopic method as well as comparison with previous literature data and chemical evidence. All the isolated coumarins were tested for AChE inhibitory activity by Ellman spectroscopic method. Of the isolated coumarins, gosferol was showed strong inhibitory effect against AChE with IC50 value of 8.3 mM. In addition, xanthoarnol and marmesin were also found to be active in inhibiting AChE with IC50 values of 10.2 and 13.3 mM, respectively. Of coumarin glycosides, feronielloside was the most active AChE inhibitor with IC50 value of 16.9 mM.