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Biological properties of polysaccharide gel (PG) extracted from fruit-hulls of durian (Durio zibethinus L.) were studied to evaluate its resistance to enzyme alpha-amylase activity and lipid entrapment property, in vitro study was performed. Powder of PG swelled and formed a viscous layer in water, 2%PG solution showed 279.36+-25.87 cps viscosity. PG showed its resistance to alpha-amylase digestion and PG was only partially digested by the enzyme, alpha-helical structure of PG disappeared according to iodine solution test; trace amount of reducing sugar without monosaccharides end product after alpha-amylase digestion was received according to the O-toluidine test compared to maltose standard TLC technique. PG was also demonstrated resistance against hydrolysis in dilute hydrochloric acid. Lipid entrapment property of PG was investigated in vitro by using semipermeable membrane dislysis technique. Lipids, cholesterol, oleic acid and stearic acid; were determined using PG at 0-2% concentration and bile salt being used as an addition surface active agent to help solubilize lipid in water to make homogeneous solution. Lipids in solution inside and outside dialysis membrane were analyzed by HPLC technique after 4-16 hours of dialysis. Increasing trapped lipids inside membrane and decreasing released lipids outside membrane were found with respect to increasing PG concentration. 2% PG trapped about 80-90% cholesterol. This result was found comparable to standard polysaccharide glucomannan. PG viscosity was also effected lipids trapping in PG, trapping of lipids in PG increased with respected to increasing viscosity of PG. PF trapping of cholesterol in egg yolk was also studied, the similar result was obtained. In vitro studies of cholesterol releasing from mixture of cholesterol with PG through out the membrane of dissected rat jejunum was performed by using membrane dialysis technique. The similar result was also obtained, increasing concentration of PG resulted in decreasing released cholesterol. The results indicated that PG has an effect to decrease lipids releasing through rat wall, according to this study, PG has expected to be used in diet food preparation. In vitro study of the effect of dietary fiber on lipid absorption by using semipermeable membrane dialysis may be used in application as a preliminary evaluation of polysaccharide influence lipids absorption.Biological properties of polysaccharide gel (PG) extracted from fruit-hulls of durian (Durio zibethinus L.) were studied to evaluate its resistance to enzyme alpha-amylase activity and lipid entrapment property, in vitro study was performed. Powder of PG swelled and formed a viscous layer in water, 2%PG solution showed 279.36+-25.87 cps viscosity. PG showed its resistance to alpha-amylase digestion and PG was only partially digested by the enzyme, alpha-helical structure of PG disappeared according to iodine solution test; trace amount of reducing sugar without monosaccharides end product after alpha-amylase digestion was received according to the O-toluidine test compared to maltose standard TLC technique. PG was also demonstrated resistance against hydrolysis in dilute hydrochloric acid. Lipid entrapment property of PG was investigated in vitro by using semipermeable membrane dislysis technique. Lipids, cholesterol, oleic acid and stearic acid; were determined using PG at 0-2% concentration and bile salt being used as an addition surface active agent to help solubilize lipid in water to make homogeneous solution. Lipids in solution inside and outside dialysis membrane were analyzed by HPLC technique after 4-16 hours of dialysis. Increasing trapped lipids inside membrane and decreasing released lipids outside membrane were found with respect to increasing PG concentration. 2% PG trapped about 80-90% cholesterol. This result was found comparable to standard polysaccharide glucomannan. PG viscosity was also effected lipids trapping in PG, trapping of lipids in PG increased with respected to increasing viscosity of PG. PF trapping of cholesterol in eggyolk was also studied, the similar result was obtained. In vitro studies of cholesterol releasing from mixture of cholesterol with PG through out the membrane of dissected rat jejunum was performed by using membrane dialysis technique. The similar result was also obtained, increasing concentration of PG resulted in decreasing released cholesterol. The results indicated that PG has an effect to decrease lipids releasing through rat wall, according to this study, PG has expected to be used in diet food preparation. In vitro study of the effect of dietary fiber on lipid absorption by using semipermeable membrane dialysis may be used in application as a preliminary evaluation of polysaccharide influence lipids absorption. |
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