Abstract:
An allelotype analysis, a method to identify the genomic regions containing candidate tumor suppressor genes was performed in Epstein-Barr-Virus-associated nasopharyngeal carcinoma (NPC). The losses of heterozygosity (LOH) on all autosomal arms using short tandem repeat polymorphic markers (STRPs) were tested after DNA amplification by the polymerase chain reaction (PCR). Twenty three NPC patients were examined for DNA from 23 primary tumor samples copaired with their own leukocytes. Eighty nine STRP markers distributed through all autosomal chromosome arms were used. In all 23 cases, chromosome arms 3p and 9p were demonstrated LOH 65.20% and 77%, while of llq, 13q, 14q, and 20q were 36.40%, 38%, 37%, and 30%, respectively. In addition, chromosome arms 1p, 6p, and 17p exhibited LOH between 20-30% From this study, it may conclude that LOH occurs significantly at least 9 loci during NPC carcinogenesis. Thus, at least 9 tumor suppressor genes are possibly involved with the evolution of NPC.
