Acid leaching extraction technique for determination of metals in fish and mussel by atomic spectrometry

Abstract

The use of diluted acid to extract lead, chromium, cadmium, copper and zinc from seafood was a solid-liquid extraction procedure with atomic absorption spectrometry. Ultrasonic-assisted acid leaching extraction technique was attempted for the multi-elemental analysis of seafood (mussel and fish) samples. The effects of several parameters: nitric acid concentration, hydrochloric acid concentration, hydrogen peroxide concentration, acid solvent volume, sonication time and temperature have been studied. A 30-minute sonication, 56 degree celcius operating temperature and 6 mL of 3.7 M HNO3/3.7 MHCI/0.5 M H[subscript2]O[subscript2] of 1:1:1 ratio as acid solvent were used. The results obtained by the proposed procedure were evaluated by comparison with the results obtained by microwave-assisted digestion. %Ratio of metal amount obtained from leaching technique to amount determined by digestion technique for cadmium, copper and zinc ranged from 92-114% andfrom 88-103% for fish and mussel samples, respectively. Whilst, chromium and lead were not leachable from both samples by this condition. The MDL were 0.08, 0.02, 0.13 and 0.63 mg/kg for lead, cadmium, copper and zinc, respectively. The accuracy of the developed method was test by analyzing DORM-2, Dogfish Muscle certified reference material. Found values from acid leaching technique and digestion technique of cadmium were higher than certified values. %Recoveries for zinc and copper were in the order of 80 to 87%