DEVELOPMENT OF B/Z-DNA NANOBIOSENSOR FOR DETECTION OF Z-DNA BINDING PROTEINS

Abstract

The left-handed Z-DNA has been attempted to elucidate the biological significance since several research studies suggest correlation between the Z-DNA and pathogenesis of many diseases, for example, systemic lupus erythematosus, rheumatoid arthritis, and Alzheimer's disease. Because protein–DNA interaction can control various biological processes, studying of the interaction between Z-DNA and Z-DNA binding proteins is the key to understand biological role of Z-DNA. In this study, B/Z-DNA nanobiosensor was designed and developed to determine the binding of Z-DNA forming probe to specific Z-DNA binding proteins under surface plasmon resonance (SPR) technique detection. Z-DNA forming probe was designed to contain the Z-DNA facilitator, 5-methylcytosine, in an alternating cytosine-guanine tract. The circular dichroism results have demonstrated the designed Z-DNA forming probe could convert to the left-handed conformation and specifically bind to Z-DNA specific binding protein. After B/Z-DNA biosensor development, DNA hybridization study and DNA-protein binding study on surface were investigated. All SPR results have presented the complete of DNA immobilization process and the B/Z-DNA biosensor can hybridized specifically to complementary target. This study provided the optimal SPR condition for DNA hybridization study, nevertheless, the SPR condition for study of DNA- protein binding on biosensor surface needs further optimization.