Bf2-curcumin-based and anthraquinone imidazole-based sensors for detection of nucleotides

Abstract

A new fluorescence sensor (L1), based on BF2-Curcumin and dpa (bis (2-pyridylmethyl)amine) for detection of Zn2+and Cu2+ ions was synthesized. The sensor demonstrates the blue-shifted absorption band from 472 nm to 421 nm and the fluorescence quenching at 587 nm in acetonitrile after adding both metal ions. The dpa-Zn complex was expected to be used as chemosensor for detecting biological nucleoside polyphosphates in aqueous solution. Unfortunately, in aqueous system two Zn2+ ions upon dpa-Zn complex were released by water. Therefore, we developed the chemosensor containing pyrene and dpa-Zn complex (L2•Zn) for detection of nucleotide in aqueous solution. This sensor has a high selectivity for PPi in aqueous solution. In contrast for the case of DMSO:HEPES (0.01 M, pH 7.4 90:10 v/v), L2•Zn selectively bind to nucleoside polyphosphates such as ATP and UTP with a large fluorescence enhancement. The Principal Component Analysis (PCA) was used to discriminate between 10 nucleotides by two solvent systems. This approach was found to identify 10 analytes with 88% accuracy. To illustrate the utility of this approach for ATP hydrolysis application, the ratio between the product of reaction (PPi) and the reactant (ATP) can be used in quantitative identification of the PPi and ATP ratios.