ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF PEPTIDE FROM SPOTTED BABYLON Babylonia areolata

Abstract

Inappropriate free radical levels are considered to be one of the major causes of various syndromes in living systems. Oxidative stress, which occurs when free radicals and antioxidant levels are in excess and insufficient, respectively, leading to cell and tissue damage, can be alleviated in some circumstances by the consumption of additional antioxidants. In this study, peptide derived from spotted babylon (Babylonia areolata) was hydrolysed using three kinds of proteases (alcalase, neutrase and flavozyme), and the flavozyme hydrolysate obtained by 3-h hydrolysis exhibited the highest DPPH, ABTS, NO, SOD, Catalase and GPx activities assay compared to other hydrolysates. By using ultrafiltration and gel filtration chromatography, the F3 fraction from the flavozyme hydrolysate of spotted Babylon was highest antioxidant activity. After that, F3 fraction from gel filtration chromatography was tested in RAW 264.7 cells by inhibition of NO production assay and MTT assay, and the F3 fraction was purified using HPLC technique. The chromatogram showed the profile which contained 4 main peaks. These 4 fractions will analyzed using mass spectrometry which was obtained 6 sequence in 4 peak.