Investigation of total antioxidant status and antioxidant activity in pre-dialysis chronic kidney disease patients

Abstract

Chronic kidney disease (CKD) has still been a worldwide public health problem including Thailand. Oxidative stress is considered as an important pathogenic mechanism in CKD. Evaluating the status of micronutrients that have antioxidant properties along with energy and proteins intake, is extremely important to combat antioxidant deficiencies, minimize oxidative stress, and improve the overall health status. This study aimed to measure and compare oxidative stress, antioxidant markers and their relationships with dietary antioxidant food pattern. This study was a cross-sectional study and was performed in outpatients Metabolic clinic. Thirty-three Thai pre-dialysis CKD outpatients and 13 healthy participants were recruited. All patients and controls completed a food frequency questionnaire. Then, anthropometric measurements, general biochemical and antioxidant lab tests were performed. Malonaldehyde (MDA), Total antioxidant status (TAS), Glutathione peroxidase (GPx), Paraoxonase 1 (PON-1) and dietary antioxidant food pattern score were investigated. The results showed that MDA significantly increased (p = 0.01), and PON-1 significantly decreased among CKD patients and controls (p <0.05). There was a positive correlation between the rich-vitamin C vegetable pattern with plasma GPx. Based on this study indicated that oxidative stress increase and antioxidant enzyme activity decrease in Thai CKD outpatients. Therefore, the assessment of dietary antioxidants was important, as deficiency of these nutrients might influence on the balance of the oxidative stress and antioxidants in CKD patients.