HOST RESPONSES IN PERI IMPLANT TISSUE IN COMPARISON TO PERIODONTAL TISSUE

Abstract

The host response in peri-implant tissue may differ from that in periodontal tissue in a healthy individual. The purpose of this study is to investigate the expression of inflammatory cytokines in peri-implant crevicular fluid (PICF)from single implant with two different abutment types in comparison to healthy periodontal tissue. Moreover, the effect of these inflammatory cytokines on the expression of FAK and RANKL were also studied. Nineteen participants with healthy implants and teeth were recruited according to the inclusion and exclusion criteria. PICF and gingival crevicular fluid (GCF) was collected using sterile paper points. The expression level of inflammatory cytokines including IL-1α, IL-1β, IL-6, IL-8, TNF-α, IFN-y was assessed using enzyme-linked immunosorbent assay (ELISA). varying concentrations of PICF obtained around two abutment materials were treated with human gingival fibroblast cell cultures to study the expression of FAK and RANKL using qPCR. Expression of IL-6, TNF-α and IFN-y was not statistically different from GCF among titanium and UCLA abutment group. However, the level of IL-1α in the PICF from the implants restored with UCLA abutment was significantly higher than GCF (p- value= 0.030). Inaddition, the level of IL-1β in PICF from implants restored with titanium abutment was significantly higher than GCF (p-value=0.032). IL-8 expression in PICF from implant restored with UCLA abutment was significantly higher than titanium abutment ( p-value 0.003). Moreover an up- regulation of RANKL and down regulation of FAK gene was found to be in a concentration dependent manner for both the abutment groups.