An in vitro virucidal efficacy of disinfectants against porcine circovirus type 2

Abstract

The objective of this study was to evaluate in vitro the virucidal activity against PCV2b of nine commercial disinfectants which were divided into three groups consisting of oxidizing products, QAC and iodine products, and two chemical substances including an alkali agent and aldehyde agent. Each disinfectant was freshly diluted in distill water and hardness of water as conducting the disinfectant test procedure. Five percent of fetal bovine serum were added as organic matter. After mixing the disinfectant with the stock of PCV2b, the final concentrations were 0.5x, 1x, 2x of the products’ labelled dilutions. After 1, 10, 30-minute and 12-hour contact time between the virus and the disinfectant, the mixture was detoxified by flowing through a detoxification column. The infectivity of the collected virus was determined by indirect immunoperoxidase monolayer assay and indirect immunofluorescence assay. Viability of PCV2b was illustrated after 12-hour exposure to QAC products (disinfectants 6 and 7) and iodine products (disinfectants 8 and 9). PCV2b inactivation was found after 30-minute contacting with a combination of glutaraldehyde and QAC (disinfectants 4 and 5). Oxidizing products were the most effective disinfectants (disinfectants 1, 2 and 3) against PCV2b after 10-minute contact time. Of oxidizing products, a combination of potassium peroxomonosulfate and sodium dichloro isocyanurate (1:200), which significantly reduced PCV2b titer (5 log10TCID50ml-1) after 1-minute exposure, indicated the best virucidal product against PCV2b.