Abstract:
Many infectious diseases have been well recognized caused by several genotypes of human enterovirus species which significantly in public health. Publications worldwide have reported on the emerging and re-emerging of enteroviruses. Until now, epidemiological surveillance data, clinical complication and evolutionary history regarding these viruses in actual on the Asia continent are still limited. To address these concerns, this study aimed to establish comprehensive population-based surveillances, and provided evidence for the evolution in many diseases such as acute haemorrhagic conjunctivitis, herpangina, hand foot mouth disease, acute gastroenteritis, poliovirus and meningitis caused by enteroviruses. Employing reverse transcription-polymerase chain reaction and sequencing approaches for detection, our study revealed that each enterovirus has a specific to cause different disease. Results displayed that infections by coxsackie A24 caused acute haemorrhagic conjunctivitis, enterovirus species A caused herpangina and hand foot mouth disease, enterovirus species B caused meningitis, various of enterovirus species associated with acute gastroenteritis. These represent the surveillance data of multiple enteroviruses circulate in Thailand. Moreover, this study also investigated the evolutionary relationship based on the analysis of the VP1 gene of poliovirus vaccine strain and meningitis. For poliovirus surveillance, this study did not found positive selective pressure and evolution rate is 3.7x10-4 s/s/y which lower than rate of vaccine derived poliovirus strain. This study supported that transition of oral to inactivated poliovirus vaccine is effective and nearly complete eradication. For meningitis cases, the evolution rate is 1.33x10-3 s/s/y. The phylogeographic analysis revealed that this virus transmitted from Europe and did not found strain from Asia. This study revealed the potential of etiologic agent and transmission pattern that circulated worldwide.
