The use of modified mRNA encoding platelet-derived growth factor-BB as an innovation in periodontal regeneration

Abstract

Since the mRNA platform has been recently introduced to several fields in medicine and the achievement of periodontal regeneration is currently unpredictable, therefore, the use of mRNA technology is a promising approach to pursue the complete periodontal regeneration. The aims of this study are to learn if mRNA encoding platelet-derived growth factor-BB (PDGF-BB) induces PDGF production in human periodontal ligament cells (PDLCs) and to investigate the effect of secreted PDGF on PDLC proliferation and promoting endothelial cell tube formation. PDLCs were obtained from extracted teeth of healthy periodontal patients. The N1-methylpseudouridine modified mRNA encoding PDGF-BB were transfected into PDLCs. The supernatants were collected from 24-, 48- and 72 h and measured the protein production using ELISA assay. PDLCs were also tested for cell viability. In addition, the supernatants collected at 48 h and 72 h were used for proliferation assay and induction of endothelial cell tube formation, respectively. The results showed that PDLCs transfected with mRNA encoding PDGF-BB, produced higher level of intracellular PDGF-BB than the control at 24 h. PDGF-BB was detected in the supernatants started from 24 h and constantly secreted up to 72 h. The transfection of PDLCs by mRNA had no effect on the cell viability. The supernatants containing secreted PDGF-BB were able to promote PDLC proliferation and endothelial cell tube formation. In conclusion, modified mRNA encoding PDGF-BB promotes PDGF-BB production both intra- and extracellularly. Moreover, the supernatants containing PDGF-BB induce PDLC proliferation and endothelial cell tube formation. Thus, mRNA platform technology is possibly applicable for periodontal tissue regeneration.