Abstract:
Purifications of human ceruloplasmin and haptoglobin were employed, using ion exchange and affinity chromatography. The purified proteins were used to immunize adult rabbits for 2 to 4 weeks. The rabbit antisera were made monospecific to respective proteins by immunoadsorption with suitable serum proteins. The antisera obtained were used in quantitative determinations of serum ceruloplasmin and haptoglobin in patients who suffered from liver diseases, and hemoglobinopathies. Serum ceruloplasmin was increased in case of liver diseases. Serum haptoglobin was decreased in case of hemoglobinopathies.