Abstract:
Lignocellulosic biomass can be used to produce fermentable sugar, which is used in the production of biofuels. However, enzymatic action is hindered by the recalcitrance of plant cell walls. Among the chemicals used in the pretreatment of biomass, acid can give high glucose yield; however, it can also form inhibitors. To solve these problems, a new method is being used to improve the total sugar concentration by adding acid as a catalyst during ionic liquid pretreatment. This research focused on a suitable composition of Napier grass, the effect of acid type, acid concentration, pretreatment time, biomass loading and temperature during ionic liquid pretreatment. The optimal condition of these variables were determined by response surface methodology (RSM) for producing maximum reducing sugar. The result showed that maximum reducing sugar concentration of 14.38 g/1 was derived when Pakchong l_Kanchanaburi (Leaf) was pretreated by l-Ethyl-3- methylimidazolium acetate and acetic acid at 147℃, 76 min, acid 1.287 volume% and biomass loading of 20 g/1.