Influence of surface morphology to the protein adsorption on polycaprolactone film

Abstract

Protein adsorption is the first phenomena that would occur when the foreign materials are inserted into the body. The materials used in biomedical application can have different surface topology. It is important to know the effect of surface to protein adsorption. The main objectives of this study were to prepare the polycaprolactone (PCL) film of different surface topology and different degree of crystallinity. The protein adsorption on various surface characteristics was studied. Surface of polycaprolactone was made to have different topology by different phase separation using solvents of various solubility parameters. The solvents applied in this study were chloroform, acetone, tetrahydrofuran (THF) and ethanol. The degree of crystallinity was varied by different annealing time. Surface of polycaprolactone film could be modified for better protein adsorption by aminolysis, using 1,6 hexamethylenediamine (HMD). Then, the amino groups were activated by N, N’disuccinimidyl carbonate (DSC) before the immobilization of protein called bovine serum albumin (BSA). The PCL films of different surface topology and the protein adsorbed PCL films will be studied for their degree of crystallinity, hydrophobicity, functional group on the surface, surface roughness and cytotoxicity. It was found that degree of crystallinity does not affect the amount of protein adsorbed. The result from atomic force microscope (AFM) showed that the film casted from 40:60 (v/v) EtOH: THF had the roughest surface and the protein assay proved that it had significantly higher amount of protein adsorbed. The potential use of the film was evaluated by mouse-calvaria derived pre-osteoblastic cells (MC3T3-E1). The indirect cytotoxicity test showed that the materials were not harmful to the cells and cells proliferated best on the roughest surface