Abstract:
The purpose of this study was to find out for the optimal technique to prepare plasma with highest platelet concentration in platelet rich plasma (PRP), as well as determine the result of autologous PRP from the dogs with osteoarthritis(OA) condition. It was shown that the PRP could reduce inflammation, cell regeneration, and enhance angiogenesis to support the injured tissue. This study was composed of two phases; the first phase was aimed to find the best centrifugation speed and time that could be able to produce the plasma with the highest platelet concentration by conducting the experiment from a total of 6 healthy dogs without OA condition, which was verified by physical examinations and blood tests. The blood from these dogs was collected with Arthrex® Double syringe. In the second phase, a total of 10 dogs with OA condition in both side of coxofemoral were used to perform the experiment by intraarticular PRP injection. In addition, a video double blinded clinical evaluation on a pre-PRP and a post-PRP injection state were performed, which was evaluated by radiographic finding, lameness score, kinematic data analysis (Kinovea®), and owner questionnaire (CBPI/LOAD) at the day before PRP and post PRP injection at 8 weeks. The PRP would be processed by using the centrifugation speed and time results in the first phase. The result of the first phase indicated that using 1,500 rpm centrifugation speed for 3 minutes given the highest platelet concentration and significant in terms of statistics, and the result of the second phase revealed that lameness score, kinematic data analysis, and the owner questionnaire post PRP injection at 8 weeks were resulted in significantly improved in terms of statistics. However, there were not significantly improvement for the results of the radiographic findings. In conclusion, using PRP from the autologous blood of the dog given an improved clinical effectiveness for the dog with OA condition. Nevertheless, the frequency of injection, as well as the appropriate container, and the centrifugation speed and time will be required to a further study.
