Development of electrospun nanofiber modified disposable electrode for biosensor applications

Abstract

A new type of biosensor was fabricated using a carbonized hybrid gold (Au)/graphene (G) nanowire and vapor-phase polymerization of conductive polymer constructed on a disposable screen-printed carbon electrode (SPCE). Electrospinning, carbonization and conductive polymeric coating processes were combined to achieve the selective and sensitive determination of biomolecules such as glucose (GU) and dopamine (DA). Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used to characterize the surface morphology and physical properties of the electro spun products. The basic characterization of electrochemical behavior of the various modified electrodes in [Fe(CN)6]3-/4- was studied by cyclic voltammetry (CV) and Electrochemical impedance spectroscopy (EIS). The results show that modified electrodes exhibited drastically high current response compared to unmodified electrode. Moreover, the various methods of electrochemical studies; amperometry (APM), square-wave voltammetry (SWV) and differential pulse voltammetry (DPV) were used to systematically measure and optimize the oxidation current of biological analyses. Finally, the linear current response to biological concentrations, sensitivity, limit of detection (LOD) and interfering studies. The modified electrode could be a promising candidate for use as a high-potential electrode, representing a new approach for the selective and sensitive determination of biological products with long-term sensor stability.