Development of novel antibacterial wound dressing coated with calcium alginate loaded garcinia mangostana extract

Abstract

At the present, gauze is a most popular dressing, but traditional gauze is a non-create moist environment, non-antibacterial and non-blood coagulation material. Therefore, in this research, calcium alginate used to enhance traditional gauze properties; swelling ratio, moist environment and blood coagulation. The calcium alginate was used to coat on gauze by dipping gauze in sodium alginate solution and calcium chlorine solution. According toexperiment, the swelling ratio increased with the increase of calcium alginate concentration However, the coated gauze with more than 1% w/v of alginate showed a rough surface. On the other hand, the oxygen permeability decreased with the increase of concentration. The blood coagulation was also investigated by estimating blood clotting index (BCI). The BCI value was found decrease when increase the alginate concentration. Furthermore, antibacterial prosperity of gauze dressing was enhanced by mangosteen extracts. The antibacterial efficacy of mangosteen extracts was studied by using disc diffusion method (ATCC 147), the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC) and bacterial reduction method. We found that initial traditional gauze cannot inhibit all bacteria and pure calcium alginate cannot completely against all bacteria. Thus, a small of mangosteen extract was added to improve the antibacterial properties. The results showed none of bacteria was found after 24 hours in a bacterial reduction method and the increase of clear zone in a disc diffusion method. However, the increase of mangosteen extracts displayed toxicity on human cell in cytotoxicity testing.