Free radical scavening and antiproliferation of protein hydrolysate from spotted babylon Babylonia areolata prepared by pepsin-pancreatin and papain

Abstract

The world is a heavily polluted environment which affects health and is the cause of many diseases, especially cancer. Cancer is difficult to treat, and there are often adverse side effects for the patient. These diseases produce more free radicals than the body can inhibit which cause damage to molecules, DNA, cells, and body organs. Antioxidants in the body are beneficial for health. This research investigated the preparation of peptides from spotted babylon snails protein hydrolysates by plant proteases and the simulation of the human digestive system with the effect of antioxidant activity. The protein hydrolysate had the highest antioxidant activity (spotted babylon at 0.075 g/ml digested by pepsin-pancreatin); through a ultration system with five separate molecular weights. The antioxidant activity was tested by three methods, DPPH, ABTS, and NO radical scavenging assays. It was found that the molecular weight of protein 3 kDa had the highest inhibition antioxidant activity (IC₅₀ values of 0.918 ± 0.097, 0.502±0.075 and 0.420±0.069 µg/ml respectively.). The purification and identification of characteristics from protein hydrolysates of spotted babylon showed eight peptides. In addition, results indicated inhibition in the growth of cancer cells for five species which were tested for apoptosis of cancer cells. It was found that protein hydrolysate at 3 kDa produced the highest antiproliferative of the growth of lung cancer cells (CHAGO-K1), with less apoptosis compared to doxorubicin which is an anticancer drug used in medicine.