The effect of molecular weight of PLGA on DEX-P loaded PLGA nanoparticles on encapsulation efficiency

Abstract

Biodegradable polymer is one of the most suitable polymers for drug delivery system in human body. One of these polymers is poly (D,L-lactide-co-glycolide) (PLGA), which has properties like biodegradability, biocompatibility, and non-toxicity. Magnetic PLGA nanoparticles were synthesized by the water-in-oil-in-water (W/O/W) emulsion technique using poly (vinyl alcohol) as a surfactant. Dexamethasone Phosphate (DEX-P) was using as a model drug. The concentration of drug in the water inner phase was varied using and low molecular weight of PLGA in order to study the concentration of drug on loading efficiency and release profile. In this study, magnetization and magnetite loading to PLGA nanoparticles were determined by a vibrating sample magnetometer (VSM) and thermogravimetric analyzer (TGA), respectively. Particle size and the aggregation of magnetite (Fe₃O₄) in PLGA nanoparticles were evaluated by TEM. Moreover, the zeta potential of PLGA nanoparticles was determined by dynamic light scattering technique. High Performance Liquid Chromatography (HPLC) was used and studied the drug loading and the drug release profile of the loaded sample.