Abstract:
This study was to assess the expression of Bcl2a1 and Cxcl9 genes in soft tissues covering bone grafted with demineralized freeze-dried bone allograft (DFDBA) and deproteinized bovine bone mineral (DBBM) in comparison with ones covering without grafting. The animals were randomly separated into three groups of treatment. Calvarial defect models were created on parietal bones. Bare defect models without bone graft as a control group. After one and three months, animals were sacrificed. Soft tissues covering the defected area were collected by punch technique. RNA was isolated and proceeded to Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Statistical analysis indicated that in comparison to control group at one month, DFDBA and DBBM upregulated Bcl2a1 gene in soft tissue covering bone graft at P < 0.05. Allograft also upregulated Cxcl9 gene (P < 0.05). However, the comparison among groups at three months and comparison within group between one and three months shown the expression of both genes but without significant difference (P > 0.05).
