Abstract:
This study aimed to isolate secondary metabolites from the stems of Abutilon indicum and to evaluate their α-glucosidase, pancreatic lipase, α-chymotrypsin inhibitory activity. Chromatographic fractionation of the n-hexane crude extracts led to the isolation of three known metabolite (1-3). The crude extract from all parts of the plant were evaluated for inhibitory activity on the alpha-glucosidase enzyme. The DCM-soluble roots were active with 10.2% at 25 μg/mL. The hexane and MeOH-soluble stems were active with 11.0% at 25 μg/mL and 8.0% at 0.25 μg/mL. The hexane, DCM, and MeOH-soluble leaves are active with 20.2% at 0.025 μg/mL; 20.0% at 0.025 μg/mL; and 14.6% at 2.5 μg/mL, respectively. Furthermore, the phytochemicals of A. indicum were studied for its interaction against α-glucosidase, pancreatic lipase, α-chymotrypsin through molecular docking. Based on the in silico study, ADME investigation, and toxicity prediction, nine compounds (2, 8, 11, 12, 13, 15, 17, 18, and 19) out of 66 compounds were potential to be developed as novel inhibitor for α-glucosidase, human pancreatic lipase, and α-chymotrypsin. The result demonstrated that isolated compounds from A. indicum potential to be anti-diabetes, anti-obesity, and anti-ulcer.
