Abstract:
Objectives: This study aimed to evaluate the genes of interest that could explain osteoblast and osteoclast activities in bone remodeling after bone grafting with demineralized freeze-dried bone allograft) (DFDBA) and demineralized bovine bone mineral (DBBM) to compare with normal bone healing.
Methods: Calvarium defects were created on both side of the parietal bone in nine male C57BL/6MLac mice. Defects were divided into 3 groups; group 1: defect without grafting as a control group, group 2: defect grafted with DFDBA, and group 3: defect grafted with DBBM. The mRNA expression levels of MMP12 and S100A4 were analyzed using real-time PCR in 1month and 3 months.
Results: In month 3, the expression of S100A4 gene was significantly increased, compared with MMP12 gene in both DFDBA and DBBM groups. Moreover, the expression of MMP12 and S100A4 genes was significantly increased in both DFDBA and DBBM compared to the control group. In DFDBA group, the expression of MMP12 gene was significantly decreased in month 3 compared to month 1.
Conclusion: The DFDBA and DBBM promoted bone remodeling in month 3. In addition, DFDBA has properties to help bone formation better than DBBM.
