Antiproliferative agents from mushroom phellinus sp.

Abstract

In this study, Phellinus powdered (600 g) was extracted with methanol and DI water. The methanolic crude extract of the mushroom was partitioned with hexane, dichloromethane, ethyl acetate and methanol respectively. All crude extracts were evaluated antiproliferative activity against lung cancer (A549) and neuroblastoma (SH-SY5Y) cell lines by MTT assay. It was found that the dichloromethane extract (CDE) exhibited the strongest antiproliferative activity with half maximal inhibitory concentration (IC50) values of 132.4 and 106.87 µg/mL against human lung cancer and neuroblastoma cell lines, respectively. The ethyl acetate extract (CEE) exhibited moderate antiproliferative activity with IC50 values of 138.64 and >250 µg/mL against SH-SY5Y and A549 cell lines, respectively. The hexane extract, methanol extract and aqueous extracts from reflux extraction and decoction exhibited weak antiproliferative activity against both cancer cell lines with IC50 >250 µg/mL. Isolation by repeated column chromatographic, followed by NMR characterization, ergosta-7,22-dien-3-one and 7-methoxyindole-3-carboxylic acid methyl ester, 7-methoxyindole-3-carboxaldehyde and protocatechualdehyde from hexane extract, dichloromethane extract and ethyl acetate extract, respectively.