องค์ประกอบทางเคมีและแอกทิวิตีทางชีวภาพของผลิตภัณฑ์ผึ้งจากผึ้งโพรง (Apis cerana) และชันโรง (Tetragonula laeviceps)

Abstract

In this research, it was focused on the anti-proliferation of cancer cells and the anti-agglutination of human blood cell infected by H1 N1 virus (AHB) of honey and AHB of propolis from Tetragonula laeviceps. Honey (90 g) was extracted by 96% EtOH and H20 resulting in CHE and CHW, respectively, Later, it was tested against the proliferation of 5 cancer cell lines (BT474, Chago, Hep-G2, KATO-III, and SW620) and CH-liver as normal cell by MTT assay. The data was estimated from the average of percentage of cell viability (PS) and statistically analysed by SPSS statistics 17.0. The result showed that PS of CHE and CHW were closed. After partition, CHH gave the best activity. However, after being purified by quick column chromatography, 6 fractions contained no activity. Furthermore, for AHB, hemagglutination inhibition assay was used. It presented that both CHE and CHW had no AHB activity. In addition, after partition, all CHM, CHD, and CHH still had no ABH activity. Instead, propolis was focused. Both CPE and CPW still had no ABH activity. Thus, it could be concluded that 1) anti-proliferation compounds had synergistic effect to each other so more purification decreased the activity. 2) Crude extracts of bee products from T. laeviceps had no ABH activity at all.