Abstract:
Recently, a number of alternative source including the bioactive compounds from plant and fungi are necessary to explore as therapeutic against cancer. This study aims to investigated the antioxidant and anticancer activities of Smilax sp. and Phellinus linteus (P. linteus) crude extract individually, also their mixture ratio variation including combination 1 (Smilax sp. 50%: P. linteus 50%), combination 2 (Smilax sp. 25%: P. linteus 75%), and combination 3 (Smilax sp. 75%: P. linteus 25%). The individually extract and its mixtures were investigated the physiochemical characterization such as total phenolic content and total polysaccharide compound. Furthermore, the antioxidant activities of extracted samples were determined by DPPH and ABTS assays. The anticancer activities on three distinct breast cancer cell lines as well as cytotoxicity, antiproliferative, colony formation, and apoptosis. The individually Smilax sp. extract revealed a high level of total phenolic content, while individually extract of P. linteus had a great amount of total polysaccharide content. The total phenolic content and total polysaccharide content of all combinations were relied on the relative ratios of individual extract content. The antioxidant activities showed the higher value through DPPH and ABTS assays were individual Smilax sp. and combination 3, followed by combination 1, combination 2 and P. linteus. The toxicity results of the extracted samples on breast cancer cell lines were related and have the same trends to its antioxidant activities. Moreover, certain combinations exhibited a greater inhibitory effects on cell proliferation against breast cancer than both individual extracts. Similar to cell proliferation, some combinations showed the greatest effect to reduce the form of colonies compare to individual extracts. However, the apoptosis induction results were not potent due to the value less than 5%. In conclusion, the individual extracts and in combination have their capabilities as antioxidant and anticancer agent.
