Abstract:
The objective of this study is to study the effects of cereal supplementation, which contains many nutritional factors including amino acids on renal functions and morphological alteration in 5/6 nephrectomized rats. Spraque-Dawley rats, 250-300 g, were use in this study. Rats were divided into 4 groups. The first group has been undergone sham operation (SOR). Group 2, 3 and 4 undergone 5/6 nephrectomy by removed the right kidney and ligated 2/3 of renal artery. After surgery rats were kept in the individual cage for 5 weeks. Rats in group 1 and 2 (CNR) fed with normal protein diet only. Rats in group 3 were fed with normal protein diet and supplemented with 0.03 g/rat/day cereal (LNR). Rats in group 4 were fed with normal protein diet supplemented with 0.06 g/rat/day cereal (HNR). The cereal supplement was started 1 day after surgery. After 5 weeks rats were anesthetized and subjected to renal function study. At the end of the experiments, left kidney was isolated for the measurement of morphological alteration. There was no significant difference of body weight, blood pH, blood pressure and heart rate among all groups of rat post-treatment. However, rats with cereal supplementation had significant higher (P<0.05) plasma urea and plasma creatinine concentration than rat without cereal supplementation. Rats in CNR had slightly higher in GFR and RPF compared to the left kidney from SOR indicating functional compensatory. Rats in LNR groups had similar GFR and slightly lower RPF compared to CNR causing higher filtration fraction. Rats in HNR group had higher GFR and RPF than CNR group. The higher GFR caused urinary sodium excretion in HNR higher than in CNR while fractional excretion of sodium was similar. The higher urinary flow rate caused urinary potassium excretion slightly higher in HNR compare to CNR while fractional excretion of potassium was similar. The structural changes that observed were similar among all groups of nephrectomized rats. In conclusion, 0.03 mg/rat/day cereal supplement did not improve renal function while 0.06 mg/rat/day cereal supplement increase GFR and ERPF in 5/6 nephrectomized rats 5 weeks after treatment. Different levels of cereal supplement did not show any differnce on renal structural alteration compared with nephrectomized alone.
