Molecular epidemiology of human bocavirus and new discovery human bocavirus 2

Abstract

The recently discovery virus base on metagenomic technique, human bocavirus (HBoV), had been screening in this study. Nasopharyngeal (NP) aspirate and stool from acute diarrhea patients and healthy children were corrected. The result showed that HBoV can be detected in NP aspirate 6.62% and diarrhea stool samples 0.9%. Where as could not be detected in healthy children stool samples. The co-infection with other respiratory viruses also found (40%) and there were no clinical specific manifestations for HBoV; however, fever and productive cough were commonly found. The results from complete coding sequences showed that the most conserved regions of HBoV are the NS1 and NP1 genes, whereas VP1 and VP2 showed frequent variations. The epidemiological study of newly discovered species of Bocavirus genus, HBoV2, which show about 74% amino acid identity when comparing with HBoV had been also focusing in this study. HBoV2 could be only found in diarrhea stool samples that collected in both Thai (0.61%) and UK patients (0.64%). Moreover, it has shown higher variation at the genome level than HBoV. In conclusion the molecular epidemiological study of HBoV and HBoV2 provided more information of these viruses.