Secondary metabolites of endophytic fungus isolate are-1 from Annona reticulata leaf

Abstract

In this study we investigated for secondary metabolites of the endophytic fungus isolate ARE-1 from Annona reticulata L. (Annonaceae) leaf. Chromatographic techniques were used to isolate compounds from the YES culture broth and mycelia of the endophytic fungus isolate ARE-1. Five known compounds were isolate and identified as succinic acid monoethyl ester, phenylacetic acid, 2-(4’hydroxy) ethyl acetate, 4-hydroxyphenethyl alcohol and ergosterol, a primary metabolite that is a major component of fungal cell membrane. The chemical structures of the isolate compounds were elucidated through extensive analyses of UV, IR, MS and NMR spectroscopic data and comparison with literatures. Ergosterol exhibited weak antituberculosis activity with MIC value of 12.5 µg/ml. Based on conventional method, the fungus isolate ARE-1 limited in spore formation. Nucleotide sequencing of ITS1-5.8S-ITS2 sequences of rDNA was applied to classify the endophytic fungus isolate ARE-1. It was found to be in the family Valsaceae with evolution closely related to Diaporthe caulivora.