Determination of bisphenol-a-diglycidyl ether, bisphenol-f-diglycidyl ether and their derivatives in oil-in water and aqueous-based canned foods maufactured in Thailand

Abstract

A new method for simultaneous identification and quantification of bisphenol- A-diglycidyl ether (BADGE), bisphenol-F-diglycidyl ether (BFDGE), and their derivatives in foods is described. Analyses were carried out using RP-HPLC gradient elution with fluorescence detection. Extraction for oil-in-water foods used tert-butyl methyl ether as solvent but changed to acetonitrile for aqueous foods. The developed method was validated for the analyses for both types of samples. The quantitation limits ranged from 2 to 23 ng/g of food depend on compounds. Satisfactory method efficiency was evaluated from recovery and intra-and intermediate precision data. The method was used to investigate the presence of BADGE, BFDGE, and their derivatives in 15 types of oil-in-water canned foods and 10 aqueous types, all manufactured in Thailand. The level of contamination was determined by HPLC and reconfirmed types of internal coatings by Beilstein’s test. Analysis results of 80 cans found 41% contaminated above the limit of 1 mg/kg restricted by the European Union. This report also presented the preparations and characterizations of the unavailable standard reference materials: BFDGE.H2O, BFDGE.HCl.H2O, and BFDGE.HC1.