Immunization against ascariasis : an experimental study in Hanoi Medical School

Abstract

Prospects for vaccine against the most widespread and serious nematode infections in man are not very encouraging. Vaccination against Ascaris with secreted larval has been reasonably successful in some experimental animal. In this study an attempt was made to find functional antigens of Ascaris Suum and Ascaris lumbricoides that induce protective immunity in mice. For this purpose in vitro cultures of both L2 and L3/4 were set up. An experimental model to test the immunogenicity of the antigens has fist to be establish before protection studies could be carried out. Challenge infections with embryonated eggs of Ascaris Suum and Ascaris lumbricoides were carried out to test for functional antigens Where as the ELISA was used to follow the humoral responses-cross protection was studied with Ascaris lumbricoides material in order to eliminate future problems with regard to availability of human material. Both somatic and E/S antigens of L2 and L3/4 of Ascaris Summ and Ascaris lumbricoides induce protection immunity measured as reduced numbers of migrating larvae.