Selective immuno-detection of amphetamine and/or methamphetamine by principle of heterologous combinations

Abstract

The application of heterology principle for immuno-detection of amphetamine and methamphetamine was studied. N-(3-aminopropy1)amphetamine(A), N-(4-aminobuty1)amphetamine(B), N-(3-aminopropy1)methamphetamine(C) and N-(4-aminobuty1)methamphetamine(D) were synthesized for preparing immunogen and labeling with peroxidase enzyme. The reaction that amphetamine and methamphetamine could compete with HRP-labeled in binding to antiboly by heterologous pattern was determined. Amphetamine and methamphetamine could be selectively determined using the combinations of antiserum and enzyme-labeled from (C) with the sensitivity of competitive binding equal to 34 per log concentration. Ephedrine, pseudoephedrine and phenylpropanolamine could cross-reacted with amphetamine in the relative reactivity of 0.27, 0.14, respectively. Selective determination of amphetamine, methamphetamine and ephedrine was resulted from the combinations of antiserum from (C) and enzyme-labeled from (A). The sensitivity of competitive binding equal to 25 per log concentration. Pseudoephedrine and phenylpropanolamine could cross-reacted with methamphetamine in the relative reactivity of 0.05 and 0.10, respectively. These two types of combinations were selective for both amphetamine and methamphetamine. Therefore, amphetamine and methamphetamine could be successfully determined using polyclonal antibody and heterology study. The further immunoassay development was suggested before utilizing in drug-abused test in Thailand