THE FACTORS EFFECT TO EXISTENCE OF METHICILLIN-RESISTANT COAGULASE POSITIVE STAPHYLOCOCCI (MRCoPS) ON DOG SKIN AND AN ANIMAL HOSPITAL

Abstract

Coagulase-positive staphylococci (CoPS) is common pathogenic bacteria distributing in veterinary hospitals. Methicillin-resistant coagulase-positive staphylococci (MRCoPS) is the important resistant trait and can cause zoonotic infection in human associated with dogs. In recently years, the distribution and contamination of MRCoPS in veterinary hospitals have been increasingly concerned. In this study, we proposed 1.) to determine the increasing of MRCoPS following the routine oral treatment by cephalexin monohydrate 2.) to investigate the distribution of MRCoPS in a veterinary school hospital and 3.) to determine the bactericidal efficacy of povidone-iodine (PI) and chlorhexidine gluconate in isopropanol (CGI) against MRCoPS, in vitro. MRCoPS were isolated from all dogs treated with cephalexin (n=38) at the first week administration and methicillin-resistant S. pseudintermedius (MRSP) had persisted until at least 6 months after drug-off date (n=10). MRCoPS were the most frequent at dermatological clinic followed by gynecological clinic especially on surfaces of floor and high touch sites such as examination table and rebreathing circuit. The optimal time and concentration of PI and CGI for bactericidal effect were 0.1% for 45s and 0.5% for 15s, respectively. In this study, the factors associated the distribution of MRCoPS in veterinary hospital were the consequence of dermatitis treatment causing persistence of bacterial resistance and certain inappropriate hygienic management in equipment and environment. To select the right antiseptic at optimal time and concentration could reduce risk of bacterial resistant outbreak in veterinary hospital.