Molluscicidal activity of saponins from sapindus rarak fruits on pomacea canaliculata

Abstract

The chemical and biological study of a methanolic extract prepared from the fruits of Sapindus rarak DC. Led to the isolation of sapindoside B and mukurozi-saponin E₁. Their structures were determined by interpretation of spectroscopic data (IR, MS, 1-D NMR and 2-D NMR). Sapindoside B and mukurozi-saponin E₁ showed the molluscicidal activity with 100% mortality at 7 ppm against Pomacea canaliculata after treating 72 h. and showed the LC₅₀ values at 4.34 and 4.28 ppm, respectively after treating 24 h. The MTT colorimetric assay for cytotoxic activity of Sapindoside B mukurozi-saponin E₁ showed moderate cytotoxicity against human gastric carcinoma (KATO-3) activity level with IC₅₀ values of 5.55 and 6.17 mug/ml, respectively.