Glucose evolution from cassava residue by microbial hydrolysis using bacteria isolated from thai higher termites

Abstract

The possibility of using cassava residue from a bioethanol plant containing 49.66% starch, 21.47% cellulose, 12.97% hemicellulose, and 21.86% lignin as a raw material to produce glucose using enzymatic hydrolysis was investigated. In the experiment, each reactor contained cassava residue, bacteria cells, and production medium. The effects of particles size (40 mesh, 60 mesh and 80 mesh), strain of bacteria (A002 and M015) isolated from Thai higher termites, Microcerotermes sp., reaction temperature (30 C and 37 C), and concentration of secondary carbon source on the sugars concentration were tested. High performance liquid chromatography was used to determine the quantity of sugars. The maximum sugar concentration obtained at 30C using strain A002 and 80 mesh cassava residue was 1.44 g/L at 7 h. The optimum quantity of malt extract was 10g/L.