Co-electrodeposition of gold nanoparticle and reduced-graphene oxide in application of achetylcholinesterase biosensor

Abstract

A rapid pesticide detection technique is highly desirable due to the widespread usage of many pesticides in the agricultural industry. An emerging technique that shows promising potential is an electrochemical biosensor. The technique allows sensitive and rapid quantification of an analyte. Due to its simplicity, it could be a potential alternative to traditional method. In this work, we utilised a composite materials consist of partially reduced graphene oxide (rGO) and gold nanoparticle (AuNPs) to fabricate a disposable AChE biosensor on a Screen-Print Carbon electrode. The composite rGO and AuNPs possess high electron conductivity while AuNPs also exhibit desirable biocompatibility. The fabricated biosensor was used to detect an organophosphate pesticide called Chlorpyrifos. The observed linear ranges of the fabricated biosensor was 0.016mg/mL to 1mg/mL and 1mg/mL to 25 mg/mL. The limit of detection was 0.3503 mg/mL. The AChE biosensor was sucessfully fabricated with rGO/AuNPs as support materials.