Encapsulation of water-dispersed clove oil in alginate beads and cellulose films

Abstract

The aim of this research was to prepare of clove oil (CO) film and clove oil-loaded alginate bead to use as fish anesthetic. Both forms were based on the incorporation of clove oil in the matrix of carboxymethylcellulose (CMC) and xanthan gum (XG) to enhance the water solubility of CO in the water and seawater. The loading efficiencies of CO film and bead were 32.7% and 8.0%, respectively. The CO films dissolve in water better than that in the seawater, whereas CO-loaded alginate bead swelled faster in the seawater but released faster in the water. The SEM images, IR spectrum and TGA thermograms clearly confirmed the presence of clove oil in the network of polymer matrix. The CO film and bead were kept in vacuum and non-vacuum foil sealed bag at 40°C for 120 days. Their chemical and physical properties were investigated. The color of both forms was gradually yellow. The LC percentage of CO film was reduced but that of CO bead unchanged in both conditions. Most properties of both forms were not significantly different between storage in vacuum and non-vacuum.