Diterpenoids from the roots of Croton oblongifolius Roxb.

Abstract

The objective of this study was to search for bioactive compounds from the roots of Croton oblongifolius. The dichlomethane crude extract of the roots of C. oblongifolius was purified by chromatographic techniques to afford two new compounds, 19,20-dimethyl-15,16-epoxy-3,13(16),14-clerodatriene-17,18-oate-12-one and methyl-15,16-epoxy-3,13(16),14- clerodatriene-18,19-olide-17-oate and seven known compounds namely levatin, crovatin, nasimalun A, nasimalun B, 15 hydroxy-cis-ent-cleroda-3,13(E)-diene, patchoulenone and (-)-hardwickiic acid. The chemical structures of all isolated compounds were established on the basis of chemical and spectroscopic methods. The isolated compounds were also subjected to bioactivity test. Patchoulenone exhibited cytotoxicity against P388 cell line with IC[subscript 50] value of 1.06 [microgram]/ml. 15 Hydroxy-cis-ent-cleroda-3, 13(E)-diene, nasimalun B and methyl-15,16-epoxy-3,13(16),14- clerodatriene-18,19-olide-17-oate showed weak cytotoxicity against the T47D cell line with IC[subscript 50] value of 17.0,13.0,and 10.0 [microgram]/ml, respectively.