Estrogenic-like compounds from some Pueraria candollei varieties and cell culture

Abstract

The tuberous roots of five varieties of Pueraria candollei were collected from Kasetsart University, Kampangsaen Campus and cell cultures that were established from the tuberous root tissue of Pueraria candollei collected from Udon Thani province were studied for their estrogenic-like activity by using of estrogen dependent cancer cells (MCF-7 cells). The cell cultures were established on MS medium with 2,4-D, BA and NAA (1: 0.1: 0.1). The crude extracts of some varieties of Pueraria candollei showed similar chemical patterns whereas some showed different pattern. The cell cultures appeared to produce two major isoflavonoids, daidzein and genistein. Boronate affinity chromatography was developed for separation of active ingredients from the ethyl acetate crude extracts and methanol crude extracts of some varieties and cell cultures of Pueraria candollei. MCF-7 cells were used for detection of estrogenic activity of unbound and bound fraction. The compound that has R[subscript f] value 0.4 on TLC plate could bind to the boronate resin (adsorbents) in alkaline solution and eluted by decreasing pH with acid. This compound can found in all samples