Development production of amino acid and organic acid from lignocellulosic materials by Corynebacterium sp. isolated from Thailand

Abstract

The samples were collected from buffalo dung in Suphanburi province (SP-I, SP-II), soil sample in Suphanburi province (SP-III) and cow dung from Surin province (SR-I, SRII), Thailand. Thirty-three isolates with ability amino acid and thirty-two isolates with ability succinic acid were obtained. They were non-spore-forming, gram-positive or negative rod. The morphological characteristics colonies appearing on the screening agar plate after 24 h of incubation were circular, smooth and gray with 1–2 mm in diameter. Three of those isolates have a potential for succinic acid production (isolate SP-II/A1, SP-II/A3 and SP-II/A4). Corynebacterium glutamicum DSM20300 was used as a representative for investigating growth and succinic acid production from agriculture biomass. The use of nitrogen source, combination of 10 g/l of peptone and 10 g/l of yeast extract, gave the highest amount of cell growth of 9.62 g/l. Using glucose as a carbon source exhibited better cell growth than using monosugar from SSS hydrolysate. A maximum cell growth of 14.34 g/l was obtained with 10 g/l of glucose. In case using SSS hydrolyzate as a carbon source, longer lag phase was found and the highest amount of cell growth of 9.82 g/l was obtained.