Effect of thermal process on bioactive compounds in crinum asiaticum leaves

Abstract

Crude extracts from heated-Crinum asiaticum Linn leaves by 2 processes including steaming (95 ºC, 1 h) and oven drying (160 ºC, 10 minutes) compared with crude extracts of fresh Crinum asiaticum Linn leavevs. Steamed leaves and oven dried leave gave the lycorine content, which is important bioactive compound in Crinum asiatiucum Linn leaves were 3.69±0.04 and 4.48±0.02 mg/mL, respectively. While lycorine content of the fresh leaves was 2.35±0.09 mg/mL, which oven dried leaves gave lycorine content approximately to 2 fold of fresh leaves. Therefore, heated-Crinum asiaticum Linn leaves by oven-drying process were chosen for study the effects of various temperatures (50, 70, 100 and 120 ºC) and times (10, 30, and 60 minutes) on bioactive compounds in Crinum asiaticum Linn leaves. Oven dried Crinum asiaticum Linn leaves of temperature at 50 ºC for 30 minutes was the highest lycorine content. All crude extracts were tested antioxidant activities that the heated leaves at 50 ºC for 30 minutes and 100ºC for 10 minutes showed the greatest antioxidant activity. Moreover, all crude extracts were tested cytotoxic activities against 5 cancer cell lines including SW620, CHAGO, KATO-III, Hep-G2 and BT474 by MTT assay. Crude extracts of oven dried-Crinum asiaticum Linn leaves of temperature at 50 ºC for 30 minutes showed high cytotoxic activity against gastric cancer cell line (KATO-III), IC50 was 1.5 ± 0.26 µg/mL. Moreover, Crude extracts of oven dried Crinum asiaticum Linn leaves of temperature at 50 ºC for 30 minute were non-toxic to lung normal cell line (WI-38) at 50 µg/mL. Furthermore, we studied the effect of crude extracts from Crinum asiaticum Linn leaves for anti-inflammatory. For 0.1 µg/mL, The heated Crinum asiaticum Linn leaves of 50 ºC for 30 minutes and 100ºC for 10 minutes showed the greatest inhibited inflammation, which is more than the parthenolide compound. While the fresh leaves exhibited minimal effect. Therefore, heated-Crinum asiaticum Linn leaves by oven-drying process especially oven dried leavevs of temperature at 50 ºC for 30 minute had effect to increased lycorine content which has efficacy for antiantioxidant and against gastric cancer cell line. Moreover, crude extracts of oven dried Crinum asiaticum Linn leaves can helpful to develop for anti-inflammatory.