Electron microscopic observation of anti-hepatoma monoclonal antibodies on human hepatocellular carcinoma

Abstract

Nine of anti-hepatoma monoclonal antibodies (anti-hep MAbs) were purified by affinity chromatography and tested for the tumoricidal effect. From this series, the MAb #27 and #43 were selected for further study. The MAb #27 at ug per 1x10⁴ cell showed the inhibition of proliferation on HCC-S102 and HepG2 cell with 62% and 70% viability on day 2 after treatment, respectively. The MAb #43 at 5 ug per 1x10⁴ cell showed antitumor effect on HCC-S102 and HepG2 cell with 78% and 59% viability on day 2 after treatment, respectively. The MAb #43 at 10 ug per 1x10⁴ cell showed antitumor effect on HCC-S102 cell with 40% viability on day 4 after treatment. Morphologic studies showed that MAb #27 had early effect direct to rough endoplasmic reticulum. The number of ribosome were decreased after 3 hours of treatment. Mitochondria was swollen and the number was parallely decreased. The MAb #43 had effect to the distorted and swollen mitochondria. Surface antigens of HCC-S102 cell recognized by anti- hepatoma MAb #27 were decreased after the treatment for 3 hours, which was found in cytoplasm. The number of these antigen still decreased until 12 hours after treatment. The antigen was totally can not found after treatment 24 hours until day 3. The results from this study suggested that anti-hepatoma MAbs are effective to some hepatoma cells in pattern of time and dose dependent. The MAb #27 and #43 showed some different route of attaching, despite of the early attach to the protein transportation system.