Lipoic acid-incorporated calcium carbonate nanoparticlesfor controlling of adipose derived stem cells (ADSCs) differentiation and adipocyte function

Abstract

CaCO3 NPs has received a growing interest due to its potential properties as a safe drug carrier. The LA-CaCO3 NPs were synthesized by a chemical precipitation method and characterized using transmission electron microscopy, thermogravimetric analysis, and zeta potential measurement. We used LA-CaCO3 NPs to evaluate their therapeutic effects in lipid accumulation, lipolysis, adipogenesis, and adipokines expression in ADSCs and differentiated adipocytes. The average particle sizes were observed to be 10-20 nanometers. Thermogravimetric analysis results confirmed the percentages of LA in CaCO3 NPs. The zeta potential showed a negative charge -30.9 mV. Our findings indicated that LA-CaCO3 NPs were apparently more effective in lipid accumulation and lipolysis than LA alone. Additionally, LA-CaCO3 NPs were also inhibit adipogenesis in ADSCs as well as adipokine expression.LA-CaCO3 NPs directly reduced lipid accumulation by enhancing of lipolysis activity. Adipogenesis was also suppressed by LA-CaCO3 NPs and eventually decreases in adipokine expression. The LA-CaCO3 NPs with beneficial therapeutic effects could provide a promising approach to target adipose tissue for obesity interventions.