Investigation of oxygen in red blood cells using NMR techniques / Wanjana Wannaphahoun

Abstract

In the study of relationship between spin-lattice (T1) andspin-spin (T2) relaxation times and dissolved oxygen, T1 and T2values of phosphate buffer saline solutions (PBS) were measured.The result indicated that only T1 was oxygendependent. Theresults from measurement of T1 and T2 of the dissolved oxygensolutions containing different quantity of red blood cells atsome measured times indicated that T1 depended on oxygenconsumption of red blood cells and the quantity of red bloodcells while T2 depended on the quantity of red blood cells.Regression-analysis, the relationship between T1 change and thequantity of oxygen consumption of red blood cells was linear andcould be presented in the form of equation and rate of spin-spinrelaxation was linear relationship with percentage of red bloodcells. Accordingly, proton relaxation times could be applied inthe investigation of oxygen consumption of red blood cells.