Abstract:
Skin transplantation or grafting is the principle choice for treating the severe skin losing injury over hundred years. Pinch and punch grafting which is indicated for the treatment of granulating wound, low-grade infection wound and irregular surface wound. Fibrin glue which is the combination of fibrinogen and thrombin has been developed to use as an adhesive for skin graft surgery. In this research used bubaline fibrinogen had the highest fibrinogen concentration and qualification which was extracted by cryoprecipitation and cryocentrifugation. For bubaline fibrin glue application, a healthy swine was operated two times. The first operation, parallel square areas with full skin depth wounds were created at dorsal of loin area on each side for creating the granulation wound. The second operation was done five days later for pinch and punch skin grafting. The control group was done by routine pinch and punch grafting, but the bubaline fibrin glue was added to the experimental group with before a graft was placed. There was a significant statistical difference of graft remaining, which remained graft pieces in the bubaline fibrin glue group were more than that in the control group (p<0.05) even if, wounds have been contaminated by numerous bacteria. The histological grading for skin graft rejection indicated no (grade 0) or indeterminate (grade 1) for the rejection of both control and bubaline fibrin glue group. The angiogenesis, fibroblast proliferation, inflammation and scarring of skin graft in both groups found that there was a statistical significant difference of the number of new microvessels between two groups on 3rd post-operative day (p<0.05), which the number of new microvessels in the bubaline fibrin glue group was more than that in the control group and there was a statistical significant difference of intensity of the inflammation on 7th post-operative day (p<0.05), which the intensity of the inflammation in the bubaline fibrin glue group was lower than that in the control group. From the experiment, the bubaline fibrin glue achieves advantages in the full thickness pinch and punch skin grafting due to high concentration of fibrinogen that relate to graft survival because it may not only reduce hematoma and/or seroma, movement of the graft but also promote the angiogenesis.
