Abstract:
This study assessed the effects of blood contamination on the chemical composition and ion release of calcium silicate-based materials. ProRoot MTA (WMTA), Biodentine and TotalFill BC RRM putty (TRRM) were investigated. Materials were exposed to blood (blood-contaminated condition) or normal saline (non-blood-contaminated condition) for 24 hours. Three samples of each group were analysed for chemical composition using X-ray diffractometer and an energy-dispersive X-ray spectoscope with a scanning electron microscope after immersed in phosphate-buffered saline for 1, 7, 14 and 28 days. Five samples of each group were used to measure pH and ion release using a pH meter and inductively coupled plasma-optical emission spectrometer after immersed in deionized water for 1, 7, 14 and 28 days. Apatite formation was found in blood-contaminated groups later compared with non-blood-contaminated groups. In both conditions, WMTA demonstrated apatite formation earlier than the other materials. Silicon ion release was reduced in all blood-contaminated groups. Blood contamination delayed apatite formation and decreased silicon ion release on the surface of calcium silicate-based materials.
